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For the quantitative assessment of gene regulatory evolution, different types of regulatory sequences can be interrogated that can be assessed in different functional assays, mostly using the chromatin immunoprecipitation and sequencing (ChIP-seq) technology. For example, these can be RE-linked transcription factor binding sites (TFBS) in the gene promoter or enhancer regions. Alternatively, this can be done also for the histone modification tags that correlate with the structural chromatin alterations . For all of those types of regulatory elements, the RetroSpect method makes it possible to calculate relative and absolute metrics of RE-linked regulatory enrichment for both individual genes and the whole intracellular molecular pathways (D. Nikitin et al., Cells 2019). In RetroSpect, relative retroelement enrichment scores for individual genes are reflected by NGRE value (normalized gene RE-linked enrichment score). For the assessment of molecular pathways, NPII (normalized pathway involvement index) values are calculated which reflect relative retroelement enrichment scores for the whole molecular pathways . dN/dS approach was added to characterize in parallel the regulatory and structural evolutionary profiles for the genes and molecular pathways. Totally, the evolutionary metrics are available for 10,890 individual genes and 2,972 molecular pathways.